Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Inhibition of matrix metalloproteinase expression by selective clearing of senescent dermal fibroblasts attenuates ultraviolet-induced photoaging.

doi: 10.1016/j.biopha.2022.113034

Figure Lengend Snippet: Fig. 2. Selective elimination of senescent cells in UV-irradiated mice skin after treatment with ABT-263 or ABT-737. Hairless mice were exposed to chronic UV irradiation for eight weeks with 100 mJ/cm2 for the first 2 weeks, then 50 mJ/cm2 weekly, then 300 mJ/cm2. Each drug (ABT-263, 5 μM; ABT-737, 10 μM) or vehicle (DMSO) was diluted in PBS (30 μl) and injected intradermally into the dorsal skin for a total of ten doses. (A) Representative images of SA-β-gal staining in mice skin. SA-β-gal (blue color) was detected in 6 µm frozen skin sections from unirradiated control mice injected with vehicle (n = 7, white bars), UVB-irradiated mice injected with vehicle (n = 4, black bars), UVB-irradiated mice injected with ABT-263 (n = 6, blue bars) or ABT-737 (n = 7, red bars). Arrowheads indicate stained cells. (B) Quantitative analysis of the percentage of SA-β-gal positive cells in the indicated mice skin (n = 3–4 mice per group) (C) Western blots and quantification of p21 and p16 protein levels normalized to GAPDH using protein extracts from the skin. (D) p16 and p21 mRNA levels in indicated mice skin, standardized to GAPDH. (E) Representative images of immunofluorescence staining for p16 (red) and DAPI (blue) in the skin of the indicated mice. Right: Quan tification of the percentage of p16-positive cells among DAPI-positive cells in over n = 4 mice per group. Arrowheads indicate stained cells. Data represent means ± SEM, *p < 0.05, * *p < 0.005, * **p < 0.001. p-values were calculated by Student’s t-test. A, Bar = 100 µm; E, Bar = 50 µm.

Article Snippet: The antibodies used included p16 (MA5–17142, Thermo Fisher Scientific; ab189034, Abcam), p21 (556431, BD Pharmingen; ab109199, Abcam), GAPDH (CSB-PA00029A0Rb, Cusabio, Houston, TX, USA), β-actin (CSBPA000350, Cusabio), MMP-1 (Lab Frontier, Seoul, Korea), and type I procollagen (SP1.

Techniques: Irradiation, Injection, Staining, Control, Western Blot, Immunofluorescence

Journal: Journal of Medical Virology

Article Title: BK Polyomavirus Infection of Bladder Microvascular Endothelial Cells Leads to the Activation of the cGAS‐STING Pathway

doi: 10.1002/jmv.70038

Figure Lengend Snippet: Reagents.

Article Snippet: Rabbit polyclonal anti‐GAPDH (Merck).

Techniques: Virus, Staining, SYBR Green Assay

Journal: Translational Cancer Research

Article Title: Methyl vanillate for inhibiting the proliferation, migration, and epithelial-mesenchymal transition of ovarian cancer cells via the ZEB2/Snail signaling pathway

doi: 10.21037/tcr-22-2240

Figure Lengend Snippet: MV inhibited EMT process in SKOV3 by targeting ZEB2 and Snail. (A) The expression levels of EMT biomarkers, snail and ZEB2 in SKOV3 cells were quantified by image analysis of the Western blot bands. The expression of GAPDH in each group was taken as intrinsic controls, and relative expressions of each protein were calculated. (B) The expression levels of EMT biomarkers, snail and ZEB2 in HOSEpiC cells were quantified by image analysis of the Western blot bands. Values represented the mean ± SD from three independent experiments. *, P<0.05, **P<0.01 vs . MV (0 µmol/L). MV, methyl vanillate; EMT, epithelial-mesenchymal transition; HOSEpiC, human ovarian surface epithelial cell.

Article Snippet: Primary antibodies against E-cadherin (rabbit monoclonal antibody, Cell Signaling Technology; cat. no. 3195S), vimentin (rabbit monoclonal antibody, Cell Signaling Technology; cat. no. 3879T), Snail (rabbit monoclonal antibody, Cell Signaling Technology; cat. no. 5741T), ZEB2 (rabbit monoclonal antibody, Cell Signaling Technology; cat. no. 97885S), and GAPDH (rabbit polyclonal antibody; Signalway Antibody; cat. no. 21612) were incubated at 4 ℃ overnight on PVDF membranes.

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Epigallocatechin-3-gallate inhibits growth and induces apoptosis in esophageal cancer cells through the demethylation and reactivation of the p16 gene

doi: 10.3892/ol.2017.6248

Figure Lengend Snippet: Treatment with EGCG leads to increased p16 mRNA expression. (A) Results of the agarose gel electrophoresis. (B) Quantitated p16 mRNA expression levels relative to GAPDH. **P<0.01 vs. the untreated cells. EGCG, epigallocatechin-3-gallate; p16, cyclin-dependent kinase inhibitor 2A.

Article Snippet: GAPDH was blotted as an internal reference using the anti-GAPDH rabbit polyclonal antibody (cat. no. AB21612-1; 1:5,000; AbSci, LLC, Portland, OR, USA).

Techniques: Expressing, Agarose Gel Electrophoresis